Egfr Knockout cell line (MC3T3-E1 Subclone 14)

Egfr Knockout cell line (MC3T3-E1 Subclone 14) Order

Catalog#

YKO-M008

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

Egfr Knockout cell line (MC3T3-E1 Subclone 14)

Species

Mouse

Gene Name

Egfr

Gene ID

13649

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Egfr gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the MC3T3-E1 Subclone 14 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Egfr gene knockout Egfr Knockout cell line (MC3T3-E1 Subclone 14) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

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Egfr Knockout cell line (MC3T3-E1 Subclone 14)

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